Validated Stability Indicating Rp-Hplc and Hptlc Methods for the Determination of Zanamivir in Bulk and Pharmaceutical Formulation

The objective of the work search out develop a natural, exact, correct, validated establishment displaying RP-HPLC and HPTLC system for the determination of Zanamivir all-inclusive and capsule portion of drug or other consumable form. The HPLC break-up was achieved on Agilent TC C18 (2) 250 x 4.6 mm, 5 μ procession utilizing travelling phase arrangement of flammable liquid – 0.02 M phosphate safeguard, pH 5, 50:50 (V/V). The flow rate was kept nonstop at 1 ml/brief time period at range temperature. At 230 nm, UV discovery was used to measure. Zanamivir had a memory time of 3.6 proceedings. The result got accompanying the detector answer was erect expected linear in the aggregation range of 2-12 μg/ml. The pattern working TLC aluminium plates precoated with silica coagulate 60F-254 as the fixed state. The solvent method contained Chloroform: methanol: tart acid (4.5:0.5:0.3v/v) and therefore flipped through .The system was found to present compact spot for Zanamivir (Rf worth of 0.29 ± 0.02). The uninterrupted regression study dossier for the measurement plots showed good connection accompanying r2=0.9999 ± 0.0001 in the aggregation range 500-3000 ng/spot. The linearity, range, accuracy, veracity, discovery, and quantitation limitations of the submitted methods, in addition to their analytical acting, were statistically confirmed. The submitted methods keep favorably separate Zanamivir from allure degradation commodity when it was endanger differing stress settings; by way of, they were respected as direct stability-signifying methods. It is decided that this technique maybe secondhand for zanamivir portion of drug or other consumable forms and bulk drug routine control of product quality.

Author(s) Details:

C. H. Bhirud,
PRES’S College of Pharmacy (For Women), Chincholi, Nashik, India.

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Keywords: Zanamivir, reverse phase high performance liquid chromatography, high pressure thin layer chromatography, virenza capsule, method development and validation, stability indicating

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