Using of PCR Technique for Prenatal Diagnosis of Sickle Cell Disease

In present study, fetal diagnostic convenience for couples carrying sickle container gene was settled with the aim for fear that birth of affected unborn young and to calculate the nervousness of ARMS-PCR by comparing results accompanying baby’s Hemoglobin pattern on follow up HPLC. Sickle cell affliction (SCD) is common in Central India and causes important morbidity and mortality. There is a shortage of prenatal demonstrative facilities close by physically to the SCD population. This is a pilot study in our domain with the aim of establishing a fetal diagnostic facility in Central India for couples moving the sickle container gene in consideration of help them make an conversant decision about a foetus troubled with SCD, in addition to calculating the sensitivity of the polymerase chemical reaction (PCR) technique in our arrangement with follow-up souped up liquid chromatography (HPLC) of the baby’s blood sample. Follow-up HPLC was accomplished to detect baby’s Hb pattern. Prenatal disease of sickle cell chlorosis was offered in total 37 cases at a distance which individual (2.7%) fetal sample was inadequate. Total 26 (70.27%) fetuses had AS Hb genotype, 3 (8.11%) had AA Hb genotype and 3 (8.11%) had SS Hb genotype while surplus 4 (10.81%) were given AA/AS Hb genotype. All couples accompanying SS fetuses opted for MTP. Follow up HPLC was acted in 24 cases, out of which 18 (75%) were compared and 6 (25%) were mismatched. In present study nervousness of ARMS-PCR was 75%. ARMS-PCR is a simple method to be established originally for providing rapid fetal diagnosis to the couples with popular sickle container mutation. The sense of ARMS-PCR can be increased by utilizing suitable methods to detect motherly cell DNA on society can accordingly be reduced by sensitising couples accompanying the sickle container gene through proper hereditary counselling and providing the alternative of Medical Termination of Pregnancy for affected foetuses. More couples will benefit from now on if we raise awareness about the safety and productiveness of modern fetal diagnostic techniques.

Author(s) Details:

Praneeta J. Singh,
Department of Pathology, Indira Gandhi Government Medical College, Nagpur, India.

A. C. Shrivastava,
Department of Pathology, Indira Gandhi Government Medical College, Nagpur, India.

A. V. Shrikhande,
Department of Pathology, Indira Gandhi Government Medical College, Nagpur, India.

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Keywords: Sickle cell disease, prenatal diagnosis, ARMS-PCR, CVS

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