Treatment of Skin Wounds in Old Animals Using Mesenzymal Progenitor Cells and Polymer Gel Compositions

 The goal of this study was to see how successful progenitor mesenchymal stem cells were as injections and as part of a polymer hydrogel for wound healing in elderly animals.

Materials and Methods: On the backs of 9-month-old white Wistar rats, fixed-size wounds (average area of 135.8 mm2) were simulated. On the day of the modelling, MSC were extracted from a human umbilical cord and injected into the wounds once (SC group). MSC were administered externally on a regular basis as one of the components of the polymer hydrogel in other animals (Polymer sc group). Examining undamaged contralateral wounds on the opposite side of the same animal’s back was used to assess the cells’ systemic effect (control sc and Control Psc groups, respectively). The Control 0 group included the intact reference wounds. Dynamic analysis of the wound region was carried out. The collagen fibres’ maturity, the epidermal layers, and the number of fibroblasts and leukocytes in different regions of the wounds were all assessed using descriptive microscopy.

The administration of MSCs, both locally and systemically, promoted wound repair. During the acute inflammatory phase, the method and site of application had little effect on the dynamics of wound healing (up to 3 days). Polymer sc was shown to be the most effective in the end. A reduction in leukocyte infiltration in wound centres (Polymer sc and SC groups) and edges (all groups, with the greatest extent in the Polymer sc group) indicated MSC’s anti-inflammatory impact. The proliferative phase, which shows itself as increased fibroblast number and collagen production, was observed in the Control Psc group, particularly in the Polymer sc group.

Conclusion: MSC therapy improves and speeds wound healing in a variety of ways, even in elderly animals. In the Polymer sc category, the best results were achieved.

Author(s) Details:

Natalia Manturova,
Department of Plastic and Reconstructive Surgery, Cosmetology and Cell Technologies, Pirogov Russian National Research Medical University, Moscow, Russia.

Victor Stupin,
Department of Hospital Surgery no 1, Pirogov Russian National Research Medical University, Moscow, Russia.

Ekaterina Silina,
Institute of Biodesign and Modeling of Complex Systems, I. M. Sechenov First Moscow State Medical University (Sechenov University), Moscow, Russia.

Please see the link here: https://stm.bookpi.org/IDMMR-V11/article/view/6086

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