Synthetic Seed Production as a Tool for the Conservation and Domestication of Celastrus paniculatus: A Rare Medicinal Plant

Celastrus paniculatus seed oil contains sesquiterpene alkaloids, namely celapagin, celapanigin, celapanin and celastrol, which are used in the conventional medicinal method for various disorders and are over-exploited in natural environments because of their high pharmaceutical value. Due to poor germination of seeds and a lack of effective methods of vegetative propagation, it seems difficult to domesticate and commercially plant these essential medicinal plant species to meet demand. The production of a reliable and effective in vitro method for producing black oil plants for commercial use is therefore of high importance. In this research, the development of Celestrus paniculatus synthetic seeds through in vitro multiple shoot proliferation was attempted. Nodal segment explants were collected from newly emerged sprouts, sterilised on the surface and cultured in the medium of Murashige and Skoog, supplemented with various shoot induction concentrations of BAP and TDZ. Largest proliferation rate of soot; Used after encapsulation with BAP and NAA enriched sodium alginate for synthetic seed processing. Shoot tip encapsulated beads were firm, clear, round and uniform in size and easy to handle, made with 4% sodium alginate. To determine the effectiveness of encapsulated shoot tips as a propagule, the effect of growth regulators (BAP and NAA) and storage time on the germination of encapsulated shoot tips was studied. The germinated beads with 2 mg/L BAP and 0.2 mg/L NAA produced an in vitro germination percentage of 80%. After storage at 5 ° C for a duration of 8 weeks, shot tips for synthetic seeds remained green and stable. Current findings indicate that as the first step in the domestication and conservation of Celastrus paniculatus, encapsulated micro shoots (synthetic seeds) could be successfully produced. Further studies needed for domestication and conservation purposes on the rooting of micro shoots, acclimatisation and transfer of plantlets produced from synthetic seeds to in vivo conditions are required.

Author (s) Details

Dr. D. L. C. K. Fonseka
Department of Crop Science, Faculty of Agriculture, University of Ruhuna, Mapalana, Kamburupitiya, Sri Lanka.

W. W. U. I. Wickramaarachchi
Department of Crop Science, Faculty of Agriculture, University of Ruhuna, Mapalana, Kamburupitiya, Sri Lanka.

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