The differences in seed protein characteristics between four diploid rice lines and their respective tetraploids were compared. The autotetraploids had higher protein content than their parental lines, with the tetraploid of the parental line ‘Kuma’ having the highest relative increase of 63 percent. In both diploids and their tetraploid lines, alkali soluble glutelins were the most abundant protein fraction. In the seeds of tetraploids of ‘Pusur,’ ‘Kuma,’ and ‘Tougou,’ the proportion of albumin and globulin fractions decreased, whereas it increased in the tetraploid of ‘Nourin.’ The polypeptide patterns of the total seed protein extracts of diploids and their tetraploids under non-reducing and reducing conditions did not indicate any qualitative variations, with major polypeptides resolving in the range of MW 110 to 13 kDa. When compared to their diploid parents, the polypeptides of ‘Nourin’ and ‘Pusur’ tetraploids appeared darker. Densitometric scanning of SDS-gels revealed a more than 10% increase in the relative concentration of polypeptides with MWs of 78, 65, 60, 40.5, 38, 36, 29, 25, 21 and 13 kDa in ‘Nourin 4x,’ 78, 36, 21 and 13 kDa in ‘Kuma 4x,’ 78, 65, 40.5, 38, 36, 25, 21 and 21 kDa in ‘Pusur 4x As compared to their diploid parents, glutelin polypeptides of MW 25 kDa in ‘Nourin 4x,’ 21 kDa in ‘Kuma 4x,’ 36 kDa in ‘Pusur 4x,’ and 38 kDa in ‘Tougou 4x’ showed the greatest densitometric increase.

Author (s) Details

Arvinder Singh
Department of Botany, Akal University, Talwandi Sabo, Bathinda, Punjab, India.

Yogesh Kumar
Department of Botany, Kurukshetra University, Kurukshetra, India.

Bhumika Arora
Department of Botany, Akal University, Talwandi Sabo, Bathinda, Punjab, India.

View Book :- https://stm.bookpi.org/CRAS-V8/article/view/786

Leave a Reply

Your email address will not be published. Required fields are marked *