Small-Scale and Time Shortening Sample Preparation Procedure to Quantify Astaxanthin, Canthaxanthin, and β-Apo-8’-Carotenoic Acid Ethyl Ester in Hen’s Egg Yolk

 Because eggs are highly nutritious, inexpensive, and readily available, they are a very important and basic food. It is critical to improve residual monitoring of eggs in order to maintain their safety. The current chapter described a simple, small-scale, and time-saving sample preparation procedure followed by simultaneous quantification of astaxanthin (AX), canthaxanthin (CX), and (beta)-apo-8′-carotenoic acid ethyl ester (ACAEE) in hen’s egg yolk using a high-performance liquid chromatography (HPLC) coupled photodiode array (PDA) detector. The HPLC-PDA was performed on a C18 column with an isocratic mobile phase. Analytes were extracted from the sample with a handheld ultrasonic homogenizer in less than 30 minutes, purified with MonoSpin®-SI, a centrifugal monolithic silica spin mini-column, and quantified. For the three analytes, the proposed method provided average recoveries of 70.5-101.1 percent, with relative standard deviations of (le)5.0 percent. The quantitation limits for AX, CX, and ACAEE were 0.3 (mu)g g-1, 0.5 (mu)g g-1, and 1.0 (mu)g g-1, respectively. The examination of a single sample took about 30 minutes in total.

Author(s) Details:

N. Furusawa,
Graduate School of Human Life Science, Osaka Metropolitan University, Osaka, 558-8585, Japan.

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