Purification and Characterization of Cellulase from Aspergillus niger

Microbial enzyme is an important biological substance or biological molecules that are tested by living organisms that act as biocatalyst to bring out a specific biochemical reaction. Enzymes are biochemical catalysts that carry out chemical reaction to speed up the biological. Chemical activity inside or outside the cell. These are referred as “Biocatalyst”. An extracellular cellulose producing Aspergillus niger isolated from the forest soil of Courtalam Taluk, Tirunelveli District. Based on the biochemical test and 18S rRNA gene sequencing the isolate was identified as Aspergillus sp. The purification of cellulase by Aspergillus niger was carried out by DEAE cellulose and sephadox G-100.

Author (s) Details

S. Karthikeyan
Department of Botany and Microbiology, The Madura College, Madurai, India.

V. Meenakshi Sundaram
Department of Botany and Microbiology, The Madura College, Madurai, India.

S. Palpperumal
Department of Botany, Saraswathi Narayanan College, Madurai, India.

D. Kathiresan
Department of Botany, Saraswathi Narayanan College, Madurai, India.

S. Sankaralingam
Department of Botany, Saraswathi Narayanan College, Madurai, India.

B. Harinathan
Department of Microbiology and Biotechnology, NMSSVN College, Madurai, India.

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