Occurrence of Endocrine-disrupting Herbicides and Related Multiresidues in Natural Surface and Ground Waters from Aquifer: Control at Regulatory Levels by a Validated and Robust SPE-RP-HPLC-DAD Screening Approach


A dependable and powerful logical technique consolidating disconnected strong stage extraction (SPE) and turned around stage elite execution fluid chromatography (HPLC) with diode cluster location (DAD), has been created for the concurrent assurance of six endocrine-disruptor herbicides, in particular two parent S-triazines and their major dealkylated and hydroxylated debasement items like atrazine (AT), simazine (SI), deisopropyl-atrazine (DIA), deethyl-atrazine (DEA), hydroxyl-atrazine (H-AT) and hydroxyl-simazine (H-SI), every one of them with herbicide action as well. As ideal circumstances were chosen a Kromasil C18 section as fixed stage and a combination of acetonitrile and phosphate cradle (pH 7.0) as versatile stage in a slope elution mode and 215 nm as frequency for optical location.

A past SPE step with a polymeric cartridge (Bond-Elut ENV-1000 mg) was completed to disengage and preconcentrate the looked for analytes before chromatographic examination giving in this way a high enhancement variable of up to 3333. The streamlined disconnected SPE HPLC-DAD strategy was submitted to a broad approval convention acquiring among different boundaries, exceptionally delicate constraints of evaluation, somewhere in the range of 81 and 100 ng L-1, which were from ten to thirty-crease lower than the permitted maximal sums for regular surface waters (1 and 3 g L-1 as ready and alert levels), as per momentum European Union orders. The unwavering quality was surveyed by a comprehensive heartiness assessment in view of Plackett-Burman fragmentary factorial model followed by Youden and Steiner factual treatment. At last, the proposed technique was effectively applied to the screening of herbicides in ground and normal surface water tests from various areas in the 23th spring (Albacete, Spain), with exceptional concentration to the hydroxylated corruption items, extremely normal and whose location has been

barely tended to up until this point, regardless of their herbicide action as well, justification for why they become a significant matter of examination.

Author(s) Details:

María Jesús Villaseñor Llerena,
Department of Analytical Chemistry and Food Technology, Industrial Engineering School, University of Castilla-La Mancha, 13071, Ciudad Real, Spain.

Please see the link here: https://stm.bookpi.org/PCSR-V3/article/view/7965

Keywords: Herbicides, s-triazines, degradation products, solid-phase extraction, high performance liquid chromatography, natural surface waters, ruggedness evaluation

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