News Update on Nucleic Acids Research: June – 2019

Rapid and simple method for purification of nucleic acids.

We have developed a straightforward, rapid, and reliable protocol for the small-scale purification of deoxyribonucleic acid and polymer from, e.g., human humor and excretory product. the strategy relies on the lysing and nuclease-inactivating properties of the chaotropic agent guanidinium salt along with the nucleic acid-binding properties of silicon dioxide particles or diatoms within the presence of this agent. By mistreatment size-fractionated silicon dioxide particles, nucleic acids (covalently closed circular, relaxed circular, and linear double-stranded DNA; fiber DNA; and rRNA) may be sublimate from twelve completely different specimens in but one h and were recovered within the initial reaction vessel. sublimate deoxyribonucleic acid (although considerably sheared) was an honest substrate for restriction endonucleases and deoxyribonucleic acid ligase and was recovered with high yields (usually over 50%) from the picogram to the mcg level. Copurified rRNA was recovered virtually undegraded. subbing size-fractionated silicon dioxide particles for diatoms (the ossified cell walls of animate thing algae) allowed for the purification of mcg amounts of genomic deoxyribonucleic acid, cellular inclusion deoxyribonucleic acid, and rRNA from cell-rich sources, as exemplified for morbific gram-negative microorganism. during this paper, we tend to show representative experiments illustrating some characteristics of the procedure which can have wide application in clinical biological science. [1]

Determination of nucleic acids in tissues by pentose analysis

The procedure for the determination of nucleic acids represented during this chapter relies on the finding that nucleic acids are often separated from different tissue compounds by their discriminatory solubility in hot trichloroacetie acid. The isolated nucleic acids are then quantitated by suggests that of quantitative chemical analysis reactions involving the monosaccharide elements of the nucleic acids. The determination of nucleic acids in tissues is basically a retardant in identification. By suggests that of the extraction procedures represented within the chapter and also the quantitative chemical analysis reactions of amide supermolecule and desoxyribonucleic acid, a substantial degree of specificity is placed on the determination of those compounds. sometimes but, false results are going to be obtained, attributable to the presence of materials within the supermolecule extracts that interfere with the monosaccharide reactions. it’s emphasised that the extraction strategies represented were developed for supermolecule determinations by spectrophotometric methods. though it had been initially thought that these procedures can be directly applicable to atom work, it’s become quite clear that the separations don’t seem to be sufficiently refined for such studies. The strategies have served, however, as beginning points for different separation procedures a lot of appropriate for atom work. [2]

Solvent-accessible surfaces of proteins and nucleic acids

A method is conferred for analytically hard a sleek, three-dimensional contour a few molecule. The molecular surface envelope could also be drawn on either color formation laptop displays or time period vector lighting tricks systems. Molecular areas and volumes could also be computed analytically from this surface illustration. in contrast to most previous lighting tricks representations of molecules, that imitate wire models or space-filling plastic spheres, this surface shows solely the atoms that ar accessible to solvent. This analytical technique extends the sooner dot surface numerical formula, that has been applied in biochemistry, rational drug style, immunology, and understanding deoxyribonucleic acid base sequence recognition. [3]

Nucleic acids and analogs for bone regeneration

With the incidence of various bone diseases increasing, effective therapies are required that coordinate a mixture of assorted technologies and biological materials. Bone tissue engineering has conjointly been thought-about as a promising strategy to repair varied bone defects. Therefore, completely different biological materials which will promote somatic cell proliferation, migration, and osteoblastic differentiation to accelerate bone tissue regeneration and repair have conjointly become the main focus of analysis in multiple fields. somatic cell medical aid, biomaterial scaffolds, and biological growth factors have shown potential for bone tissue engineering; but, off-target effects and toxicity have restricted their clinical use. the appliance of macromolecules (deoxyribonucleic acid or ribonucleic acid) and nucleic acid analogs (peptide nucleic acids or fast nucleic acids), that are designed supported foreign genes or with special structures, are often obsessed by target cells to exert completely different effects like modulating supermolecule expression, commutation a missing sequence, or targeting specific gens or proteins. because of some drawbacks, macromolecules and nucleic acid analogs are combined with varied delivery systems to exert increased effects, however current studies of those molecules haven’t however happy clinical needs. In-depth studies of macromolecule or nucleic acid analog delivery systems are performed, with a selected specialize in bone tissue regeneration and repair. during this review, we have a tendency to in the main introduce delivery systems for macromolecules and nucleic acid analogs and their applications in bone repair and regeneration. At constant time, the appliance of standard scaffold materials for the delivery of macromolecules and nucleic acid analogs is additionally mentioned. [4]

Molecular Identification and Antibiotic Sensitivity Pattern of Bacteria Associated with Decomposed Domestic Food Wastes from Akure Metropolis

Aim: This analysis was designed to assess the molecular identities and antibiotic sensitivity pattern of the microorganism isolated from rotten domestic food wastes in Akure metropolis.

Methodology: Fifteen microorganism were obtained from the Department of biology, Federal University of Technology Akure. The DNA molecules of the microorganism isolates were extracted mistreatment microorganism DNA Mini-Prep Kit. The DNA extracted was amplified and sequenced mistreatment universal microorganism primers and ABI Prism DNA sequencer severally previous their nucleotides blast. The antibiotic sensitivity take a look at of the microorganism isolates was administered mistreatment plate assay.

Results: The known microorganism isolates as well as true bacteria megaterium, eubacteria difficile, B. thuringiensis, B. sphaericus, B. mycoides, B. cereus, bacteria genus asplenii, Paenibacillus macerans, true bacteria jensenii, B. badius, B. licheniformis, B. subtilis and L. delbrueckii maintained their original name once the molecular identification with the exception of C. humiferium and B. pumilus that modified to C.pseudotuberculosis and B. Cereus severally whereas 5 microorganism showed no result at the sequencing stage. The molecular techniques disclosed the strain name of the microorganism. L. delbrueckii was proof against Augmentin, antibiotic, cefriazone whereas prone to Erythrocin with a zone of inhibition (59.77±0.66 mm). C. difficile was prone to gentamycin with a zone of inhibition (13.93±0.47 mm).

Conclusion: From this study, the microorganism isolates (C. pseudotuberculosis, B. cereus, B. licheniformis, B. subtilis, and B. cereus) incontestable  multidrug resistant property. Therefore, this might represent a heavy health threat to the folks living within the atmosphere wherever the wastes square measure drop indiscriminately. [5]

Reference

[1] Boom, R.C.J.A., Sol, C.J., Salimans, M.M., Jansen, C.L., Wertheim-van Dillen, P.M. and Van der Noordaa, J.P.M.E., 1990. Rapid and simple method for purification of nucleic acids. Journal of clinical microbiology, 28(3), pp.495-503. (Web Link)

[2] Schneider, W.C., 1957. [99] Determination of nucleic acids in tissues by pentose analysis. (Web Link)

[3] Connolly, M.L., 1983. Solvent-accessible surfaces of proteins and nucleic acids. Science, 221(4612), pp.709-713. (Web Link)

[4] Nucleic acids and analogs for bone regeneration

Yuxin Zhang, Wenjuan Ma, Yuxi Zhan, Chenchen Mao, Xiaoru Shao, Xueping Xie, Xiawei Wei & Yunfeng Lin

Bone Researchvolume 6, Article number: 37 (2018) (Web Link)

[5] Ologun, O. M., Boboye, B. and Owoyemi, O. O. (2018) “Molecular Identification and Antibiotic Sensitivity Pattern of Bacteria Associated with Decomposed Domestic Food Wastes from Akure Metropolis”, Microbiology Research Journal International, 24(3), pp. 1-11. doi: 10.9734/MRJI/2018/42155. (Web Link)

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