News Update on Lipoprotein Research: Aug – 2019

A modification of the Lowry procedure to simplify protein determination in membrane and lipoprotein samples

The original Lowry methodology of macromolecule determination has been changed by the addition of atomic number 11 dodecyl salt within the alkali chemical agent and a rise within the quantity of copper salt chemical agent. These alterations allowed the tactic to be used with membrane and conjugated protein preparations while not previous solubilization or lipoid extraction and with samples containing two hundred millimeter plant product or two.5 mm EDTA. [1]

Role of oxidized low density lipoprotein in atherogenesis.

Evidence to support a very important role of aerobic  modification in mediating the atherogenicity of lipoprotein continues to grow. New hypotheses counsel mechanisms by that Ox-LDL or merchandise of Ox-LDL will have an effect on several parts of the atherogenic method, together with dilatation properties and occlusion, similarly as lesion initiation and progression itself. These ideas counsel new approaches, that together with lowering of plasma sterol, could lead on to the bar of induration of the arteries and its complications. [2]

The mysteries of lipoprotein (a)

Lipoprotein(a) [Lp(a)] may be a molecule advanced found in human plasma that mixes structural components from the compound protein and curdling systems which is related to premature coronary cardiovascular disease and stroke. it’s assembled from beta-lipoprotein (LDL) and an oversized deliquescent conjugated protein known as apolipoprotein(a) [apo(a)], that is homologous to the proteolytic enzyme proenzyme plasmin. Plasma Lp(a) concentrations vary 1000-fold between people and represent.  [3]

Lipoprotein particles in patients with pediatric Cushing disease and possible cardiovascular risks

Background: vessel (CV) complications are the foremost important explanation for mortality in adults with brain doctor sickness (CD); very little is understood regarding CV risk factors in kids with CD. activity of conjugated protein particles by nuclear resonance (NMR) qualitative analysis could be a novel technology to assess CV risk. the target of this study is to research the proton magnetic resonance supermolecule profile in medical specialty CD patients before and one year when remission. [4]

Evaluation of Triglyceride to High-Density Lipoprotein Cholesterol Ratio and Atherogenic Indices in Gestational Diabetes Mellitus

Introduction: physiological state DM (GDM) may be a common complication in maternity. lipoid (TG) to high-density-lipoprotein-cholesterol (HDL-c) magnitude relation is AN indicator of hypoglycaemic agent resistance.

Methods: polemic findings of recent studies recommend that quality and alternative doable factors might have an effect on risk of developing GDM. during this study, a hundred thirty pregnant ladies of eighteen to thirty five years recent were recruited. Demographic information furthermore as age at the time of the take a look at and aldohexose|blood sugar|glucose} levels before and when oral glucose tolerance take a look at were obtained from all subjects. TG/HDL-C ratios were considerably higher in ladies with GDM compared to controls. [5]

Reference

[1] Markwell, M.A.K., Haas, S.M., Bieber, L.L. and Tolbert, N., 1978. A modification of the Lowry procedure to simplify protein determination in membrane and lipoprotein samples. Analytical biochemistry, 87(1), pp.206-210. (Web Link)

[2] Witztum, J.L. and Steinberg, D., 1991. Role of oxidized low density lipoprotein in atherogenesis. The Journal of clinical investigation, 88(6), pp.1785-1792. (Web Link)

[3] Utermann, G., 1989. The mysteries of lipoprotein (a). Science, 246(4932), pp.904-910. (Web Link)

[4] Lipoprotein particles in patients with pediatric Cushing disease and possible cardiovascular risks
Angeliki Makri, Anita Cheung, Ninet Sinaii, Alan T. Remaley, Maureen Sampson, Meg Keil, Elena Belyavskaya, Charalampos Lyssikatos, Maria De La Luz Sierra, Constantine A. Stratakis & Maya Lodish
Pediatric Researchvolume 86, pages375–381 (2019) (Web Link)

[5] Jameshorani, M., Arefzadeh, A., Khalighinejad, P., Ranjbar, S. and Mazloomzadeh, S. (2018) “Evaluation of Triglyceride to High-Density Lipoprotein Cholesterol Ratio and Atherogenic Indices in Gestational Diabetes Mellitus”, Journal of Pharmaceutical Research International, 22(4), pp. 1-9. doi: 10.9734/JPRI/2018/41490. (Web Link)

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