News Update on Filamentous Research: Aug – 2019

Rapid preparation of DNA from filamentous fungi

We describe a general, straightforward and cheap methodology for the isolation of deoxyribonucleic acid from filamentlike fungi. ranging from freeze‐dried plant structure 01–015% by weight is isolated as high mass deoxyribonucleic acid appropriate for restriction and tying in two h. The preparation is wiped out Eppendorf tubes and permits the process of the many samples in parallel. we’ve got used the strategy with the basidiomycetes Phanerochaete chrysosporium, genus Coprinus cinereus and also the fungus fungus genus nidulans et al. have used it with Trichoderma reesei, fungus genus niger and for the isolation of deoxyribonucleic acid from tomato plants. [1]

Filamentous growth of carbon through benzene decomposition

Carbon fibres are ready by pyrolysing a combination of benzine and element at regarding 1100°C. they need been studied by high resolution microscopy. These fibres have varied external shapes and contain a hollow tube with a diameter starting from twenty to over five hundred Å on the fibre axis. They incorporates turbostratic stacks of carbon layers, parallel to the fibre axis, and organized in coaxal sheets just like the “annual ring structure of a tree”. [2]

Lysogenic Conversion by a Filamentous Phage Encoding Cholera Toxin

Vibrio cholerae, the actuating agent of infectious disease, needs 2 coordinately regulated factors for full virulence: infectious disease poisonous substance (CT), a potent cytotoxin, and toxin-coregulated pili (TCP), surface organelles needed for internal organ organisation. The structural genes for CT square measure shown here to be encoded by a thready phage (designated CTXΦ), that is expounded to phage M13. The CTXΦ ordination chromosomally integrated or replicated as a inclusion. CTXΦ used communications protocol as its receptor and infected V. cholerae cells at intervals the canal tracts of mice a lot of expeditiously than below laboratory conditions. [3]

Prediction of Filamentous Sludge Bulking using a State-based Gaussian Processes Regression Model

Activated sludge method has been wide adopted to get rid of pollutants in waste product treatment plants (WWTPs). However, stable operation of activated sludge method is usually compromised by the prevalence of thready bulking. The aim of this study is to create a correct model for timely identification associated prediction of thready sludge bulking in an activated sludge method. This study developed a state-based Gaussian method Regression (GPR) model to watch the thready sludge bulking connected parameter, sludge volume index (SVI), in such the simplest way that the evolution of SVI are often expected over multi-step ahead. [4]

Filamentous Fungal Quality of a Bovine Abattoir and Associated Water Sources in Akure, Southwestern, Nigeria

Aims: This study examined the prevalence of thready fungi in Onyearugbulem edifice effluent samples in Akure, Nigeria.

Methodology: The  abattoir’s water supply, five m aloof from animal washings, the furnace, ten m upstream, ten m downstream and a hundred m downstream were sampled between Nov 2014 and October 2015 for aerobic and anaerobic flora counts exploitation customary suggested procedures. flora isolates were known macroscopically and microscopically. [5]

Reference

[1] Raeder, U. and Broda, P., 1985. Rapid preparation of DNA from filamentous fungi. Letters in Applied Microbiology, 1(1), pp.17-20. (Web Link)

[2] Oberlin, A., Endo, M. and Koyama, T., 1976. Filamentous growth of carbon through benzene decomposition. Journal of crystal growth, 32(3), pp.335-349. (Web Link)

[3] Waldor, M.K. and Mekalanos, J.J., 1996. Lysogenic conversion by a filamentous phage encoding cholera toxin. Science, 272(5270), pp.1910-1914. (Web Link)

[4] Prediction of Filamentous Sludge Bulking using a State-based Gaussian Processes Regression Model
Yiqi Liu, Jianhua Guo, Qilin Wang & Daoping Huang
Scientific Reports volume 6, Article number: 31303 (2016) (Web Link)

[5] O. Olusola-Makinde, O., J. Arotupin, D. and C. Adetuyi, F. (2018) “Filamentous Fungal Quality of a Bovine Abattoir and Associated Water Sources in Akure, Southwestern, Nigeria”, South Asian Journal of Research in Microbiology, 1(1), pp. 1-8. Available at: http://www.journalsajrm.com/index.php/SAJRM/article/view/734 (Accessed: 29August2019). (Web Link)

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