News Update on Dental Plaque Research: Sep – 2019

Dental Plaque as a Microbial Biofilm

New technologies have provided novel insights into however plaque functions as a biofilm. Confocal research has confirmed that plaque has associate open design almost like different biofilms, with channels and voids. Gradients develop in areas of dense biomass over short distances in key parameters that influence microbic growth and distribution. bacterium exhibit associate altered pattern of organic phenomenon either as an instantaneous results of being on a surface or indirectly as a response to the native environmental nonuniformity at intervals the biofilm. bacterium communicate via tiny diffusible signalling molecules (e.g. competence-stimulating amide, CSP; autoinducer 2); CSP induces each genetic competency and acid tolerance in recipient sessile cells. [1]

Survival of Human Dental Plaque Flora in Various Transport Media

Dental plaque samples from (i) subjects with no apparent oral malady, (ii) people subjects with periodontitis, and (iii) subjects with active dental caries were collected in 3 transport media viz. a dithiothreitol poised balanced mineral salt resolution selected as reduced transport fluid (RTF), VMG II, and changed Stuart medium (SBL). The samples were spread by sonic treatment, diluted within the several medium during which they were collected, and genteel on MM10 plant product agar. The potency of the transport media within the survival of bacterial plaque flora was firm by examination the quantitative recovery (expressed as share of the initial viable count) from the specimens hold on for numerous lengths of your time. the info showed an excellent variation within the recovery of the oral microorganism flora from the plaque samples. [2]

Dental plaque as a biofilm

Dental plaque is that the various microbic community found on the tooth surface embedded in a very matrix of polymers of microorganism and secretion origin. Once a tooth surface is clean, a acquisition film of proteins and glycoproteins is adsorbate apace to the tooth surface. Plaque formation involves the interaction between early microorganism colonisers and this film (the noninheritable  enamel pellicle). To facilitate establishment of the tooth surface, some receptors on secretion molecules area unit solely exposed to bacterium once the molecule is adsorbate to a surface. after, secondary colonisers adhere to the already hooked up early colonisers (co-aggregation) through specific molecular interactions. [3]

The ultrastructure of subgingival dental plaque, revealed by high-resolution field emission scanning electron microscopy

Objectives/Aims: To explore the ultrastructure of subgingival bacterial plaque victimization high-resolution emission scanning microscopy (FE-SEM) and to analyze whether or not extracellular  DNA (eDNA) may be unreal in ex vivo samples. [4]

Comparing Salivary and Dental Plaque Content of Sodium, Calcium and Phosphate after Sucrose Ingestion between Salvadora persica Sticks and Tooth Brush Users

Dental caries may be a biofilm-dependent oral disorder, leading to its initiation and development from possible dietary carbohydrates as key environmental factors. disaccharide specially, is taken into account because the most cariogenic of all carbohydrates; the biofilm forming in its presence is characterised by low concentrations of these crucial ions concerned in de- and re-mineralization of enamel and dentin, particularly metallic element, phosphates and halide content. among this context, this gift study aimed to check Na, metallic element and Phosphate concentrations in secretion and plaque when disaccharide uptake among Salvadorapersica / Miswak mastication sticksusersand tooth brush users. Sixty and fifty 9 adult males UN agency were victimisation Salvadorapersica (Miswak sticks) and tooth brush severally were recruited within the study, excluding those that had history of smoking tobacco or smokeless  tobacco, underneath bound medications or having chronic or oral diseases as diagnosed by oral. [5]

Reference

[1] Marsh, P.D., 2004. Dental plaque as a microbial biofilm. Caries research, 38(3), pp.204-211. (Web Link)

[2] Syed, S.A. and Loesche, W.J., 1972. Survival of human dental plaque flora in various transport media. Appl. Environ. Microbiol., 24(4), pp.638-644. (Web Link)

[3] Marsh, P.D. and Bradshaw, D.J., 1995. Dental plaque as a biofilm. Journal of industrial microbiology, 15(3), pp.169-175. (Web Link)

[4] The ultrastructure of subgingival dental plaque, revealed by high-resolution field emission scanning electron microscopy
Richard Holliday, Philip M Preshaw, Leon Bowen & Nicholas S Jakubovics
BDJ Open volume1, Article number: 15003 (2015) (Web Link)

[5] Khalil, W., Sukkar, M. and Gismalla, B. (2017) “Comparing Salivary and Dental Plaque Content of Sodium, Calcium and Phosphate after Sucrose Ingestion between Salvadora persica Sticks and Tooth Brush Users”, Archives of Current Research International, 8(3), pp. 1-6. doi: 10.9734/ACRI/2017/34676. (Web Link)

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