Molecular Identification of Methicillin-Resistant and Methicillin Susceptible Staphylococcus aureus Using RAPD Indicators


The current work sought to examine the genetic relatedness and variation of methicillin-resistant and methicillin-susceptible Staphylococcus aureus strains. Gram-positive coccoid Staphylococcus aureus is a common human pathogen that can lead to a variety of serious hospital- and community-acquired infections, including sepsis, toxic shock syndrome, pneumonia, and bacteremia. The investigation used 100 local S. aureus isolates that were gathered from various clinical instances of unrelated patients. On manitol salt agar medium, all sample isolates were cultivated at 37°C, and they were all recognised at the species level using morphological traits and biochemical assays. Visually examining the banding pattern allowed for the identification of the DNA profile of the samples. The number of bands and the banding pattern varied depending on the primers. To build a two-dimensional binary matrix, the presence or absence of a band at any location on the gel was scored. Before employing the RAPD approach, genomic DNA was extracted using the boiling procedure. mec To differentiate MRSA strains without testing for antibiotic susceptibility, a gene was amplified. Our results showed that RAPD analysis can successfully be used to determine the genetic backgrounds of isolates and to distinguish strains at the intra-specific level.


Author (s) Details

Suha M. Abed
Department of Biology, College of Science, Tikrit University, Iraq.

Batol I. Dheeb
Department of Pathological Analysis, College of Applied Science, Samarra University, Iraq.

Akeel H. A. Assie
Department of Biology, College of Science, Tikrit University, Iraq.

Basim M. Khashman
National Cancer Research Center, University of Baghdad, Iraq.

Anmar Sael Hussein
College of Medicine, Fallujah University, Iraq.


View Book :-


Keywords –  Staphylococcus aureus, methicilin resistant, methicillin susceptible, random amplified Polymorphic DNA.

Leave A Comment