Molecular Identification of Fungi Isolated from Selected Varieties of Low Postharvest Physiologically Deteriorated Cassava (Manihot esculenta Crantz)

The use of microscopic technologies to further expanciate on the makeup-based taxonomy of fungi helps in the distinction of fungal species and types. The study investigated the properties of microorganisms unique from high quality cassava flours caused from low postharvest physiologically deteriorated (PPD) cassava utilizing molecular labeling. Wholesome five varieties of cassava were secondhand out of which four differences were of yellow-fleshed low PPD cassava while individual variety was of high PPD. The cassava ancestries were subjected whole operations to a degree peeling, washing, grating, important, pulverization and were dried without any delay dryer at 120°C for 8 minutes and subsequently ground into flour accompanying cyclone hammer mill bearing a screen size of 250 µm apertures. The excellence cassava flour caused was tested for total viable fungal and bacterial count; fungi unique have been further typified and identified using microscopic techniques. Characteristic fungal counts acquired were subjected to analysis of difference (ANOVA) using Statistical Package for Social Sciences (SPSS variant 25.0) and significant method were separated applying Duncan Multiple Range Test. The private and identified creature as revealed by the DNA results are Saccharomyces cerevisiae, Aspergillus oryzae, Aspergillus niger, Rhizopus stolonifera, Penicillium sp, Aspergillus oryzae and Rhizopus microsporum. Aspergillus niger’ is famous to be dependable for application in businesses (production of enzymes such as amylases, lipases, celluloses, xylanases and proteases), healing and agricultural use. Total practicable bacterial count and fungal count of the high quality cassava flours prepared from IITA-TMS-IBA-011368, IITA-TMS-IBA-070593, IITA-TMS-IBA-011412, IITA-TMS-IBA-011371 and TMEB 419 cassava assortments were within the allowable limit of the microbial load of food allowed for human use according to the Standard Organization of Nigeria and Codex Alimentarius.

Author(s) Details:

J. P. Alimi,
Nigerian Stored Products Research Institute, P.M.B. 5044, Onireke, Dugbe, Ibadan, Oyo State, Nigeria.

S. A. Ahemen,
Akperan Orshi Polytechnic, Yandev, P.M.B.-181, Gboko, Benue State, Nigeria.

J. O. Alimi,
Department of Microbiology, P.M.B. 1, University of Ibadan, Oyo State, Nigeria.

D. A. Balogun,
Nigerian Stored Products Research Institute, P.M.B. 5044, Onireke, Dugbe, Ibadan, Oyo State, Nigeria.

O. A. Oke,
Nigerian Stored Products Research Institute, P.M.B. 5044, Onireke, Dugbe, Ibadan, Oyo State, Nigeria.

Please see the link here: https://stm.bookpi.org/RAMB-V3/article/view/9764

Keywords: Low postharvest physiological deterioration,

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