Modulation of Radiation and Mitomycin C Induced Chromosomal Aberrations by Apigenin in Human Lymphocytes In vitro


The goal of this study is to examine the modulatory effects of apigenin (APG) on human cells following in vitro treatment with mitomycin C (MMC), an antineoplastic drug, or gamma radiation (3 Gy). In blood cells treated with radiation, antineoplastic agent (MMC), and APG, cytogenetic biomarkers including chromosomal aberrations (CAs), sister chromatid exchanges (SCEs), and cytochalasin-B blocked micronuclei (CBMN) were examined. Whole blood lymphocytes were cultured in vitro according to a conventional technique. Human peripheral blood cells that had received 3 Gy of gamma radiation and then underwent post-treatment with APG did not significantly differ from one another in terms of the frequency of CAs or micro-nuclei (MN). The frequency of SCEs per cell was greater in samples treated with APG compared to controls. Lymphocytes treated with MMC in the presence of APG demonstrated a significant decrease (P 0.01) in the frequency of SCEs when compared to MMC treatment alone. According to the results of the MN test, APG therapy significantly decreased the frequency of MMC-induced MN (P 0.01)

Author(s) Details:

Narinder K. Sharma,
Genetic Toxicology and Chromosome Studies Section, Radiation Biology and Health Sciences Division, Bhabha Atomic Research Centre, Trombay, Mumbai 400085, India.

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Keywords: Radiation, chromosomal aberration, cytochalasin-B blocked micronuclei, sister chromatid exchange, mitomycin C, antimutagenic, apigenin

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