Latest Research on Bacterial Isolates: Dec – 2019

Identification and antibiotic susceptibility of bacterial isolates from probiotic products

In the gift study, a complete of fifty five European probiotic merchandise were evaluated with relation to the identity and also the antibiotic resistance of the microorganism isolates recovered from these merchandise. microorganism isolation from thirty dried food supplements and twenty five farm merchandise, yielded a complete of 268 microorganism isolates hand-picked from many selective media. Counts of food supplements showed microorganism recovery in nineteen (63%) of the dried food supplements starting from 103 to 106 CFU/g, whereas all farm merchandise yielded growth within the vary of 105–109 CFU/ml. when identification of the isolates mistreatment whole-cell macromolecule identification, mislabeling was noted in forty seventh of the food supplements and four-hundredth of the farm merchandise. [1]

16S Ribosomal DNA Sequence Analysis of a Large Collection of Environmental and Clinical Unidentifiable Bacterial Isolates

Some bacterium ar tough to spot with composition identification schemes unremarkably used outside reference laboratories. 16S ribosomal DNA (rDNA)-based identification of bacterium probably offers a helpful various once composition characterization strategies fail. However, as yet, the quality of 16S rDNA sequence analysis within the identification of conventionally unidentifiable  isolates has not been evaluated with an outsized assortment of isolates. during this study, we have a tendency to evaluated the utility of 16S rDNA sequencing as a method to spot a group of 177 such isolates obtained from environmental, veterinary, and clinical sources. For 159 isolates (89.8%) there was a minimum of one sequence in GenBank that yielded a similarity score of ≥97%, and for 139 isolates (78.5%) there was a minimum of one sequence in GenBank that yielded a similarity score of ≥99%. [2]

Bacterial joint infections in England and Wales: analysis of bacterial isolates over a four year period.

Data from 1158 cases of septic inflammatory disease reported  to the general public Health Laboratory Service (PHLS) disease management Centre (CDSC) from European country and Wales over a four year|period of time|period} period (January 1990 December 1993) ar conferred. Reports wherever a microorganism organism was isolated from secretion, or wherever associate degree organism was isolated from blood cultures wherever a designation of septic inflammatory disease was reported , were examined. Reports of infection were additional common in youngsters (12.7% of infections were within the below ten age group) and also the aged (54.7% aged sixty or over), and were higher in males all told age teams except within the aged. [3]

A library of human gut bacterial isolates paired with longitudinal multiomics data enables mechanistic microbiome research

Our understanding of however the gut microbiome interacts with its human host has been restrained by restricted access to longitudinal datasets to look at stability and dynamics, and by having solely some isolates to check mechanistic hypotheses. Here, we have a tendency to gift the Broad Institute-OpenBiome Microbiome Library (BIO-ML), a comprehensive assortment of seven,758 gut microorganism isolates paired with three,632 order sequences and longitudinal multi-omics knowledge. we have a tendency to show that microorganism species maintain stable population sizes at intervals and across humans which normally used ‘omics’ survey ways ar a lot of reliable once exploitation averages over multiple days of sampling. [4]

In-vitro Antibacterial Activity and Phytochemical Screening of Psidium guajava on Some Enteric Bacterial Isolates of Public Health Importance

Aim: The study was conducted to research the medicinal drug activity of guava and its major medicinal drug constituents (Phytochemicals).

Materials and Methods: The binary compound and ethyl alcohol extracts from the leaves and stem bark of the plant was tested exploitation well diffusion technique for his or her medicinal drug activity against some members bacteria family family isolated from diarrhetic stool sample (Escherichia coli, enterics spp, Salmonella typhi and genus Pseudomonas aeruginosa). [5]

Reference

[1] Temmerman, R., Pot, B., Huys, G. and Swings, J., 2003. Identification and antibiotic susceptibility of bacterial isolates from probiotic products. International Journal of Food Microbiology, 81(1), (Web Link)

[2] Drancourt, M., Bollet, C., Carlioz, A., Martelin, R., Gayral, J.P. and Raoult, D., 2000. 16S ribosomal DNA sequence analysis of a large collection of environmental and clinical unidentifiable bacterial isolates. Journal of clinical microbiology, 38(10), (Web Link)

[3] Ryan, M.J., Kavanagh, R., Wall, P.G. and Hazleman, B.L., 1997. Bacterial joint infections in England and Wales: analysis of bacterial isolates over a four year period. British journal of rheumatology, 36(3), (Web Link)

[4] A library of human gut bacterial isolates paired with longitudinal multiomics data enables mechanistic microbiome research
M. Poyet, M. Groussin, S. M. Gibbons, J. Avila-Pacheco, X. Jiang, S. M. Kearney, A. R. Perrotta, B. Berdy, S. Zhao, T. D. Lieberman, P. K. Swanson, M. Smith, S. Roesemann, J. E. Alexander, S. A. Rich, J. Livny, H. Vlamakis, C. Clish, K. Bullock, A. Deik, J. Scott, K. A. Pierce, R. J. Xavier & E. J. Alm
Nature Medicine volume 25, (Web Link)

[5] Ali, M., Yahaya, A., Zage, A. U. and Yusuf, Z. M. (2017) “In-vitro Antibacterial Activity and Phytochemical Screening of Psidium guajava on Some Enteric Bacterial Isolates of Public Health Importance”, Journal of Advances in Medical and Pharmaceutical Sciences, 12(3), (Web Link)

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