Latest News on Klebsiella Research: Jan – 2020

Multilocus Sequence Typing of Klebsiella pneumoniae Nosocomial Isolates

A multilocus sequence typing (MLST) scheme was developed for Klebsiella pneumoniae. Sequences of seven housekeeping genes were obtained for 67 K. pneumoniae strains, including 19 ceftazidime- and ciprofloxacin-resistant isolates. Forty distinct allelic profiles were identified. MLST data were validated against ribotyping and showed high (96%) discriminatory power. The MLST approach provides unambiguous data useful for the epidemiology of K. pneumoniae isolates. [1]

Nosocomial Outbreak of Klebsiella Infection Resistant to Late-Generation Cephalosporins

Objective: to explain the epidemiology, antimicrobial susceptibility, and control of widespread ceftazidime-resistant Klebsiella pneumoniae infections during a North American hospital and circumstances that led to delayed detection.

Design: A 2-year epidemiologic, microbiologic, and clinical cohort study.

Setting: A 487-bed general hospital in ny City.

Patients and Clinical Isolates: Patient records were reviewed retrospectively and prospectively. Isolates were obtained from the Clinical Microbiology Laboratory. [2]

The real threat of Klebsiella pneumoniae carbapenemase-producing bacteria

From early this decade, Enterobacteriaceae that produce Klebsiella pneumoniae carbapenemases (KPC) were reported within the USA and subsequently worldwide. These KPC-producing bacteria are predominantly involved in nosocomial and systemic infections; although they’re mostly Enterobacteriaceae, they will even be , rarely, Pseudomonas aeruginosa isolates. KPC β lactamases (KPC-1 to KPC-7) confer decreased susceptibility or resistance to virtually all β lactams. Carbapenems (imipenem, meropenem, and ertapenem) may thus become inefficient for treating enterobacterial infections with KPC-producing bacteria, which are, additionally , immune to many other non-β-lactam molecules, leaving few available therapeutic options. Detection of KPC-producing bacteria could also be difficult supported routine antibiotic susceptibility testing. [3]

Broad and Efficient Control of Klebsiella Pathogens by Peptidoglycan-Degrading and Pore-Forming Bacteriocins Klebicins

Gram-negative bacteria belonging to the genus Klebsiella are important nosocomial pathogens, readily acquiring resistance to all or any known antibiotics. Bacteriocins, non-antibiotic antibacterial proteins, are earlier proposed as potential therapeutic agents for control of other Gram-negative species like Escherichia, Pseudomonas and Salmonella. This study is that the first report describing pore-forming and peptidoglycan-degrading bacteriocins klebicins from Klebsiella. we’ve identified, cloned, expressed in plants and characterized nine pore-forming and peptidoglycan-degrading bacteriocins from different Klebsiella species. [4]

Levels of Biofilm Expression in Klebsiella pneumoniae Isolates Exposed to Herbal Drugs

Background: there’s endless rise in antimicrobial resistance globally. Factors liable for this occurrence especially in developing countries are yet to be properly elucidated. thanks to the financial implications of antimicrobials individuals in developing countries like Nigeria resort to the consumption of herbal drugs to treat infections.

Aims: to research the amount of biofilm expressed in Klebsiella pneumoniae isolates pre-treated with herbal drugs. [5]

Reference

[1] Diancourt, L., Passet, V., Verhoef, J., Grimont, P.A. and Brisse, S., 2005. Multilocus sequence typing of Klebsiella pneumoniae nosocomial isolates. Journal of clinical microbiology, 43(8), (Web Link)

[2] Meyer, K.S., Urban, C., Eagan, J.A., Berger, B.J. and Rahal, J.J., 1993. Nosocomial outbreak of Klebsiella infection resistant to late-generation cephalosporins. Annals of internal medicine, 119(5), (Web Link)

[3] Nordmann, P., Cuzon, G. and Naas, T., 2009. The real threat of Klebsiella pneumoniae carbapenemase-producing bacteria. The Lancet infectious diseases, 9(4), (Web Link)

[4] Broad and Efficient Control of Klebsiella Pathogens by Peptidoglycan-Degrading and Pore-Forming Bacteriocins Klebicins
Erna Denkovskienė, Šarūnas Paškevičius, Audrius Misiūnas, Benita Stočkūnaitė, Urtė Starkevič, Astra Vitkauskienė, Simone Hahn-Löbmann, Steve Schulz, Anatoli Giritch, Yuri Gleba & Aušra Ražanskienė
Scientific Reports volume 9, (Web Link)

[5] Tombari Pius, M., Samuel Douglas, A., Confidence Kinikanwo, W. and Gloria Ngozika, W. (2018) “Levels of Biofilm Expression in Klebsiella pneumoniae Isolates Exposed to Herbal Drugs”, Journal of Advances in Microbiology, 12(1), (Web Link)

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