Isolation and Identification of Non Fermenting Gram Negative Bacilli: A Prospective Study


Non-fermenting Gram-Negative Bacilli (NFGNB) pathogens that have been related to nosocomial infections include Pseudomonas aeruginosa, Acinetobacter baumanii, Burkholderia cepacia complex (BCC), and Stenotrophomonas maltophilia. Previously, NFGNB were thought to be either commensals or pollutants. The purpose of this study was to identify the various nonfermenters isolated from patients admitted to our tertiary care hospital in Jammu. The lab obtained 4585 clinical specimens, including 1234 urine, 742 pus, and 1864 blood cultures collected in a brain-heart infusion broth, 110 endotracheal catheter tips, 529 CSF samples, and 115 body fluids, as well as 110 endotracheal catheter tips, 529 CSF samples, and 115 body fluids. These samples were cultivated on blood agar, chocolate agar, and Mac Conkey’s agar for 18-24 hours at 37°C. Established methodologies and traditional biochemical assays were used to identify the clinical isolates. A total of 572 of 4585 clinical specimens were found to have non-fermenting Gram-negative bacilli, resulting in a 12.40 percent isolation rate. Pseudomonas aeruginosa was the most common strain, accounting for 312 (54.54 percent), with Acinetobacter baumanii 235 (41.08 percent), Stenotrophomonas maltophilia 15 (2.62 percent), and Burkholderia cepacia complex 10 following closely behind (1.70 percent). In our investigation, thirteen isolates (86.66 percent) were sensitive to Colistin, while four isolates (26.60 percent) were susceptible to Imipenem. As a result, NFGNB has risen to prominence as a major opportunistic disease resistant to traditional antimicrobials. As a result, early detection and empirical treatment based on the institute’s local antibiogram data will reduce mortality and improve patient management.

Author(S) Details

Ruchita Mahajan
Department of Microbiology, GMC, Jammu, India.

. Neeraj
Department of Orthopaedics, GMC, Jammu, India.

ASCOMS, Jammu, India.

Bella Mahajan
Department of Microbiology, GMC, Jammu, India.

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