Investigation and Assessment of Genetic Diversity among Pleurotus spp. Isolates from Jordan

Pleurotus is considered an important genus that belongs to the Pleurotaceae family and contains the edible Pleurotus eryngii (King Oyster mushroom). In the present research, from two locations in the north of Jordan (Tell ar-Rumman and Um-Qais), 19 Pleurotus isolates were collected. The morphological characteristics of the collected isolates showed that the collected isolates were morphologically identical. In the ribosomal DNA gene of the isolated stains, nucleotide sequence analysis of the internal transcribed spacer (ITS1-5.8S rDNA- ITS4 region) and 28S nuclear large subunit (nLSU) showed that all of them share over 98 percent sequence similarity with P. eryngii. Using inter simple sequence repeat (ISSR) analysis using 18 separate primer pairs, genetic diversity among the collected strains was assessed. 141 out of 196 bands obtained were considered polymorphic using this method, and the highest percentage of polymorphism was observed using primer UBC8277. (92.3 percent) with a 70.56 percent average Polymorphism Information Quality (PIC) value. Cluster analysis revealed that, with a simple clustering centred on selection sites, the Jordanian Pleurotus isolates fell into two major clades with a coefficient of similarity values ranging from 0.59 to 0.74. The findings of the current study show that ISSR and rDNA sequencing molecular techniques will greatly help in the classification and identification of Pleurotus spp. Uh, in Jordan. A strong correlation with geographical distribution and collection sites was discovered by phylogenetic analysis. In the future, for the king oyster mushroom in Jordan, selection of appropriate techniques for large-scale development and commercialization accompanied by breeding strategies with strains of other related populations is required.

Author (s) Details

Dr. Hanan Aref Hasan
Department of Plant Production and Protection, Faculty of Agriculture, Jeresh University, Jeresh 26150, Jordan.

Ahmad Mohamad Almomany
Department of Plant Protection, Faculty of Agriculture, The University of Jordan, Amman 11942, Jordan.

Shireen Hasan
Hamdi Mango Center for Scientific Research, The University of Jordan, Amman 11942, Jordan.

Ayed M. Al-Abdallat
Department of Horticulture and Crop science, Faculty of Agriculture, The University of Jordan, Amman 11942, Jordan.

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