In-vitro Antibacterial Activity of Thymol and Eugenol against Ralstonia solanacearum Isolated from Potato


Bacterial wilt produced by Ralstonia solanacearum is a major constraint on potato production (Solanum tuberosum L.). R. solanacearum is mostly spread by infected seed stocks, although it can also be spread by farm equipment, irrigation water, and plant-to-plant through the rhizosphere from afflicted to healthy fields. At present time, no standard bactericides are known to be effective in controlling this soil-borne disease. The goal of this investigation was to see if eugenol and thymol, two key components of thyme and clove essential oils, had any antibacterial action against R. solanacearum. The pathogen was found in potato tubers that had developed bacterial wilt signs. The inhibitory effect of these drugs was investigated using disc diffusion and minimum inhibitor concentration (MIC) approaches. A combinational test was carried out using the chequerboard assay. At 100 mg mL-1, thymol and eugenol showed 27 mm and 17 mm inhibition zones, respectively. Both eugenol and thymol were found to have bactericidal properties against R. solanacearum, with MICs of 275 and 175 g mL-1, respectively. In a combinational test, an additive effect was discovered, indicating that their use together did not significantly increase inhibition. Although thymol has a lower concentration than the other, both compounds have the potential to be employed as antibiotics to treat bacterial wilt disease. In vivo application of these volatile compounds, on the other hand, presents challenges that can be overcome by encapsulating them in a suitable nanocarrier.

Author(S) Details

George Oluoch
Institute for Basic Sciences Technology and Innovation, Pan African University, Kenya.

Edward George Mamati
Department of Horticulture and Food Security, Jomo Kenyatta University of Agriculture and Technology, Kenya.

Viviene Matiru
Department of Botany, Jomo Kenyatta University of Agriculture and Technology, Kenya.

Moses Nyongesa
National Potato Research Centre, Kenya Agricultural and Livestock Research Organization, Kenya.

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