Immunodominant Epitopes Identified on the LPS from Different Enterobacteria Using Phage Display

Context: The etiological agents of intestinal infection are Escherichia coli and Salmonella. The existence of mutual epitopes between lipopolysaccharides (LPSs) of E. was shown by a previous study. O157 coli and Salmonella. Goal: The aim of this research was to identify mimotopes of shared epitopes in various enterobacterial LPSs using phage displays. Methods: We are using IgG anti-LPS from E. Coli O157 and Salmonella for the selection of M13 phage peptide mimotopes. The amino acid sequence of the mimotopes was used to synthesise peptides that were used for rabbit immunisation in turn. ELISA and Western blot assays analysed the antibody reaction of the resulting sera against the LPSs and synthetic peptides (SPs), showing that the same antibody recognised LPS sites. The reactions of human serum samples collected from the general population against the SPs and LPSs were also analysed in a complementary test.

Results: Sixty phagotopes have been selected from the last biopanning phase. The peptide mimotope amino acid sequence review showed that the S / N / A / PF motif was a typical sequence in 4 of them. SP287/3, SP459/1, SP308/3, and SP073/14 immunised rabbit serum antibodies respond against both their own peptides and separate LPSs. The Western blot test suggests a reaction of sera against both the lateral chains and the LPS cores. The human serum analysis indicates a response to the SPs and LPSs. Conclusion: The conclusion of the study is the antibody responses to S. Typhus, S. S., Urbana. Arizonae, E. Coli O157 LPS obtained from immunised rabbits with SP287/3, SP459/1, SP308/3, and SP073/14 confirms that synthetic peptides are immunogenic mimotopes of the LPSs evaluated and that they can be considered as an alternative mechanism for defence against infections caused by LPSs. E. and Salmonella. However, previous studies with animal models of coli O157 are required to confirm their protective ability. Mimotopes of LPS epitopes of Salmonella and E are the synthetic peptides designed. Coli with immunogenic capabilities. In the design of vaccines against both enterobacteria, these mimotopes may be considered for use.

Author (s) Details

Armando Navarro
Departamento de Salud Pública, Facultad de Medicina, Universidad Nacional Autónoma de México, México.

Carlos A. Eslava-Campos
Unidad Periférica de Investigación Básica y Clínica en Enfermedades Infecciosas, División de Investigación de la Facultad de Medicina, UNAM, México and Laboratorio de Patogenicidad Bacteriana, Unidad de Hemato-Oncología e Investigación, Hospital Infantil de México Federico Gómez/Facultad de Medicina, UNAM, México.

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