Identification of Low and High Erucic Acid Containing Genotype(S) in Indian Mustard Employing Molecular Markers

The presence of erucic acid in traditional Brassica oilseeds is well-regarded as an anti-nutritional concern for human consumption, as it causes harmful effects on the heart at high enough doses. As a result, there is a compelling need to limit erucic acid content and generate low-erucic-acid cultivars using conventional and/or molecular breeding methods. When there is a wide range of genetic variety and information on these genetic endowments, crop genetic enhancement can be enhanced. On the basis of molecular markers, the current study was conducted on 48 Brassica genotypes with the goal of identifying genotypes with low and high erucic acid content. A total of 50 SSR molecular markers were chosen for genomic DNA amplification in this study. Only 23 SSR molecular markers were discovered to be polymorphic. For polymorphic SSR markers, a total of 109 alleles were identified, with an average of 4.47 alleles per locus. With a mean worth of 0.68, genetic diversity ranged from 0.55 for marker Na10-D07 to 0.77 for marker BRMS-098. The markers’ polymorphism information content (PIC) values ranged from 0.51 for SSR Na10-D07 to 0.73 for BRMS-098 with an average of 0.62. The dendrogram was created, and three significant groupings emerged. The majority of genotypes were grouped according to the places they established. Genotypes with low erucic acid concentration had a higher degree of similarity and were clustered together. In the future, mustard genotypes with more genetic variability and useful features may be used in crop enhancement programmes.

Author(S) Details

Chitralekha Shyam
Department of Genetics and Plant Breeding, RVS Agricultural University, Gwalior, 474002 M.P. India.

M. K. Tripathi
Department of Plant Molecular Biology & Biotechnology, College of Agriculture, RVS Agricultural University, Gwalior, 474002 M.P., India.

Sushma Tiwari
Department of Plant Molecular Biology & Biotechnology, College of Agriculture, RVS Agricultural University, Gwalior, 474002 M.P., India.

Niraj Tripathi
Jawaharlal Nehru Krishi Vishwa Vidyalaya, Jabalpur 482004, India.

Ashok Ahuja
Department of Genetics and Plant Breeding, RVS Agricultural University, Gwalior, 474002 M.P. India.

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