Facile In vitro Glass Slide Light Microscopy Method Using Tetracycline to Visualise a Repetitive Pattern in Aerial Plant Root Tips Filamentous Network: An Advanced Study

The main purpose of this work is to show how to visualise plant root filaments using a basic light microscopy technique. In vitro experiments on freshly plucked human hair roots indicated Tetracycline’s (TE) damaging effect on soft tissue, which was severe enough to allow detection of an underlying filamentous skeleton in a prior study. In this study, TE was examined using aerial plant roots immersed in liquid Tetracycline, in a similar way to in vitro experiments. Tetracycline appears to interact with plant aerial root tissue cells, revealing an underlying filamentous network as seen in photos and video recordings. These filaments showed tetracycline biosorption, indicating that they may be made of keratin. A literature search indicated that scanning electron microscopy provided similar, albeit aesthetically distinct, presentations of root filaments. The strategy provided here could be combined with other root function research methodologies. One of the most significant advantages is that the required minimal material and equipment is limited to a light microscope, glass slides, selected biological material, water, and powder Tetracycline. Second, the rapid acquisition of data would offer researchers with a preliminary or potentially final right conclusion.

Author(S) Details

Abrahám A. Embí Sorondo
American Association for the Advancement of Science, USA.

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