The adsorption of α-chymotrypsin on plant biomass charcoal (PBC), which was prepared from plant
biomass wastes such as bagasses and dumped adzuki beans by pyrolysis, has been examined. The
PBC was characterized by SEM, specific surface area and pore size distribution. The adsorption
isotherms were successfully correlated by the Freundlich equation. The amount of α-chymotrypsin
adsorbed on PBC was dramatically dependent upon the solution pH and temperature. Maximum
amounts of α-chymotrypsin adsorbed on adzuki bean charcoal and bagasse charcoal were observed
at weak acidic and near neutral pH, respectively. The amount of α-chymotrypsin adsorbed on PBC
decreased with an increase in the concentration of salts. Plots of the amount of α-chymotrypsin
adsorbed on PBC versus temperature exhibited an optimum temperature.
Department of Applied Chemistry, Tokyo Metropolitan University, Tokyo, Japan.
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