Evaluating the Diagnostic Performance of PCR Targeting Insertion Sequence (IS6110) for the Detection of Extra-pulmonary Tuberculosis

The disease of extra-pulmonary tuberculosis remnants a challenge to control tuberculosis in spite of remarkable progress has been created in diagnostics all the while the last decade. The polymerase chemical reaction (PCR) was used in the disease of definitive extra pulmonary infection patients and to assess the efficiency of insertion order (IS) 6110-based PCR assay as distinguished to conventional liquid idea by Microbial Growth Indicator Tube (MGIT) 960 system. Patients accompanying clinically suspected extra-pulmonary TB provided 792 dispassionate specimens. There were 22 disciplined fluids, 69 pleural fluids, 240 cerebrospinal fluids (CSF), 386 endometrial tissues, 47 lymph nodes, 22 sinus samples, 1 synovial fluid, 1 fallopian tube, 2 mind abscess, and 2 ovarian blister samples among the collections. All of these dispassionate samples were cultured on MGIT 960 tubes holding Modified Middlebrooks 7H9 broth medium and Auramine O staining (FM) for acid-fast bacterium (AFB). The Mycobacterium TB insertion series IS6110’s 123 bp fragment was the target of the PCR. In our reasoning of 792 samples, we found that they were 87.5% sensitive to endometrial samples, 92.31% alert cerebrospinal fluid, 66.66% sensitive to pleural fluid, and 60% awake lymph node samples. Calculations show that the PCR IS6110 has a linked sensitivity and particularity of 85.71% and 82.91%, respectively. It is decided that PCR using IS6110 primer gained more positivity in extra-pulmonary samples distinguished to the conventional civilization method for detecting M. infection.

Author(s) Details:

R. Venkateswari,
Department of Medical Biochemistry, Institute of Basic Medical Sciences, University of Madras, Tamil Nadu, India.

B. Usharani,
Department of Biomedical Genetics, Institute of Basic Medical Sciences, University of Madras, Tamil Nadu, India.

P. Suganthi,
Department of Medical Microbiology, Institute of Basic Medical Sciences, University of Madras, Tamil Nadu, India.

M. Muthuraj,
State TB Training and Demonstration Centre, Intermediate Reference Laboratory, Government Hospital for Chest Diseases, Puducherry, India.

Please see the link here: https://stm.bookpi.org/RAMB-V2/article/view/9369

Keywords: Polymerase chain reaction, mycobacterium tuberculosis, extra pulmonary tuberculosis, insertion sequence, cerebrospinal fluid

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