Osteopontin (OPN) is a protein that has a role in bone remodelling and tissue debridement. Although the OPN protein is cleaved, it is unknown how full-length (FL)-OPN and its cleaved version carry out their biological functions in target cells. As a result, we carried out a molecular analysis of OPN in exosomes (Exo). The Exo were extracted from THP-1 macrophages that had been differentiated with phorbol 12-myristate 13-acetate (PMA) after being primed with lipopolysaccharide (LPS). Exo were also recovered from THP-1 macrophages that had been differentiated with PMA. Using the qNano multiple analyser (diameter 59–315 nm) and western blotting with a CD9 antibody, the Exo were discovered. LPS-stimulated cells made more particles than non-stimulated cells. cells that have been activated Western blot analysis was used to validate the presence of the FL or cleaved form of OPN. An ELISA method (UdOPN) was used to measure a mixture of FL and cleaved OPN, and their presence in the Exo was confirmed. After LPS stimulation, the Ud/FL ratios dropped, showing that LPS promoted FL-OPN encapsulation in the Exo. These findings imply that LPS stimulates human macrophages to produce FL-OPN, which is then cleaved in cells or by the Exo following release. These data suggest that Exo is an effective carrier for delivering OPN to target cells. It’s important to look at its role in COVID-19 infection.
Author (S) Details
Gaowa Bai
Research Institute of Health and Welfare, Kibi International University, Takahashi, 716-8508, Japan.
Takashi Matsuba
Department of Microbiology and Immunology, Division of Bacteriology, Faculty of Medicine, Tottori University, Yonago, Tottori 683-8503, Japan.
Toshiro Niki
Department of Immunology, Kagawa University, Kita-gun, Kagawa 7610793, Japan.
Toshio Hattori
Research Institute of Health and Welfare, Kibi International University, Takahashi, 716-8508, Japan.
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