Determination of Sero-Prevalence of Chlamydia Trachomatis in Sti Patients
Sexually transmitted infections are most commonly caused by Chlamydia trachomatis (STIs). If undetected and untreated, chlamydial infections can have catastrophic repercussions. Patients that are infected spread the disease to their partners. The goal of this study was to determine the prevalence of genital Chlamydia and its relationship to bacterial flora in STI patients visiting STI clinics, to identify C. Trachomatis Antigen by Immunochromatography, to detect C. Trachomatis Antibody (IgG) by ELISA, and to look for possible associations between C. Trachomatis and other bacteria.
Methods: Blood and genital discharge specimens (Endocervical and Vaginal) were collected from 226 patients using standard techniques. Bacterial flora were isolated and identified using traditional methods. An Immunochromatographic assay (Biomerieux) and an ELISA were used to check for the presence of Chlamydia trachomatis antigen and antibody in the patients (Novatech, Germany).
Inclusion bodies were found in 69/226 (30.53 percent) of the 226 patients after Giemsa staining. Candida albicans was revealed to be most frequently related with Chlamydia trachomatis (29.41 percent). By ELISA, 102/180 (55.66 percent) of the 180 samples were positive for IgG. By immunochromatographic test, 07/50 (14%) of 50 samples were positive for Chlamydia trachomatis antigen. The results of both tests were compared and contrasted.
Although tissue culture is the gold standard for detecting Chlamydia trachomatis, serological assays are far more straightforward, sensitive, and quick. The need of early laboratory identification and appropriate therapy for Chlamydia co-infection with other STIs is highlighted by co-infection with other STIs.
Atul R. Rukadikar
Department of Microbiology, All India Institute of Medical Sciences, Gorakhpur, Uttar Pradesh, India.
Department of Physiology, All India Institute of Medical Sciences, Gorakhpur, Uttar Pradesh, India.