Determination of Fourier Transform Infrared Spectroscopy (FTIR) Typing an Enterococcus sp.


The Fourier Transform Infrared Spectroscopy (FTIR) method was employed in the current study to classify Enterococcus sp. It was taken out of a hospital in an effort to provide a standardised method for identifying and classifying enterococci. Fourier transform infrared spectroscopy is a quick and affordable technique to characterise chemical cell characteristics and identify the functional groups present in the cell wall (FTIR). Based on their vibration patterns at various infrared wave numbers, functional groups in organic compounds can be identified using the well-established method of infrared spectroscopy. Due to the advent of vancomycin resistance, Enterococcus species, one of the major causes of nosocomial infections, are difficult to treat. Studies on Enterococcus isolates are essential for epidemiological research. Typing of enterococci frequently involves both genotypic and phenotypic methods. In this study, Fourier-transform infrared (FTIR) spectroscopy was used as a novel phenotypic approach for typing Enterococci. FTIR spectroscopy data were compared to results from PCR amplification of the vancomycin gene and tests for antibiotic sensitivity; the study revealed that 6 isolates were positive for the van gene (4 of VanA, 1 of VanB and 1 VanA plus VanB). VanB was shown to be susceptible, but three of VanA and VanA plus VanB were resistant to every antibiotic tested (Ampicillin, Teicoplanin, and Vancomycin). The isolates were classified into 8 groups using FTIR spectroscopy (first derivatives). The remaining 13 Enterococcus isolates were classified into 3 clusters, with 3 groups of VanA (4 isolates), 1 group of VanB (one isolate), and 1 group of VanA + VanB (one isolate). It is possible to identify certain physical and chemical features by examining the numerous molecules and organic substances that make up the cell structure. According to the study, some Enterococci bacterial species produce organic compounds that can be used to identify their species. Because of this, clinical microbiologists believe that FTIR has a lot of potential in this field.

Author(s) Details:

Mohammad I. Abu Taha,
Physics Department, Al-Quds University, Jerusalem, Palestinian Territories.

Hatem K. Eideh,
Medical Laboratory Sciences Department, Al-Quds University, Jerusalem, Palestinian Territories.

Sameeh M. Saed,
Iskander Khoury Secondary School, Bethelehem, Palestinian Territories.

Hazem Jaber,
Medical Laboratory, Al-Maqased Hospital, Jerusalem, Palestinian Territories.

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Keywords: Enterococci, antibiotic susceptibility testing, FTIR spectroscopy, PCR, VRE typing

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