Determination of Antibacterial Evaluation, Phytochemical Screening and Ascorbic Acid Assay of Turmeric (Curcuma longa)

 The microbiological susceptibility of an ethanolic extract of Turmeric (Curcuma longa) was investigated using the agar well diffusion method. The extract was found to be active against the bacteria tested, inhibiting all of them, with Shigella flexneri having the highest inhibition zone of 13.7mm and Staphylococcus epidermis having the lowest inhibition zone of 2.3mm. The values of the Minimum Inhibitory Concentration (MIC) range from 6.25 mg/ml to 200 mg/ml. Shigella flexneri had a MIC of 6.25mg/ml, S. aureus and K. pneumoniae had a MIC of 25mg/ml, E. coli had a MIC of 50mg/ml, S. epidermis, Lactobacillus, and P. aeruginosa had a MIC of 100mg/ml, and V. cholera and S. typhi had a Phytochemistry was also used to screen secondary metabolites. The ethanolic extract was tested for fourteen (14) secondary metabolites. Turmeric’s antimicrobial effect is indicated by the presence of phytochemicals such as tannins, alkaloids, phenols, steroids, flavonoids, phlobatannin, cardiac glycosides, terpenoids, triterpenes, saponin, and others. The extract contained 66.749 mg of ascorbic acid per 100 mL, which is comparable to numerous fruits advised by dieticians. Curcumin present (Rf value 0.436) has a value that is similar to that of pure curcumin. The first findings indicate that Turmeric has a high therapeutic potential in both pharmaceutical and pharmacological formulations. It is therefore recommended that further research be done on it for its use as a plant medicinal, particularly at this time when herbal medications are becoming increasingly important as antimicrobials.

Author(s) Details:

Michael Oghenejobo,
Department of Pharmaceutical Microbiology, Delta State University, Nigeria.

O. A. Opajobi,
Department of Medical Biochemistry, Delta State University, Nigeria.

U. S. Oghenejobo Bethel,
Department of Medical Biochemistry, Delta State University, Nigeria.

U. E. Uzuegbu,
Department of Medical Biochemistry, Delta State University, Nigeria.

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