In individuals with non-small cell lung cancer, metastasis is the major cause of death (NSCLC). The epithelial-mesenchymal transition causes actin cytoskeletal reorganisation, which is commonly followed by cancer cell invasion and metastasis (EMT). This study looked at the levels of expression of the actin-associated protein cofilin-1 and the key EMT component Twist-1 in NSCLC tissues. Using lung cancer tissue arrays, immunohistochemical (IHC) staining was utilised to detect 67.4 percent of tissue regions with reciprocal levels of cofilin-1 and Twist-1. This pattern of reciprocal expression was also seen in 10 of 15 NSCLC cell lines and 21 of 25 clinicopathological NSCLC tissue sections. Twist-1 levels of 80 percent and 71.5 percent, respectively, accounted for 80 percent and 71.5 percent of the reciprocal expression pattern. Normal lung tissues, lung cancer tissues in stages I and II, and NSCLC tissues with adenocarcinoma subtypes all showed this pattern. Despite the fact that cofilin-1 and Twist-1 were expressed in opposite directions in normal lung tissues and lung tumour tissues, there was a positive correlation between the two proteins. Induced cofilin-1 expression in H1299 NSCLC cells may also decrease Twist-1 expression. An online Kaplan-Meier survival analytic tool was used to make a public microarray dataset with a maximum of 1,926 NSCLC samples available. High levels of expression of both the cofilin-1 and Twist-1 genes were connected to shorter NSCLC patient survival, particularly in the adenocarcinoma subtype, according to researchers. In this study, the multivariate Cox regression model was applied. Despite the fact that further research is needed to prove the reciprocal association between cofilin-1 and Twist-1 expression levels and NSCLC survival rates, it could be a valuable indicator of NSCLC progression.


Author (s) Details

Chun-Yuan Chang
Department of Biomedical Imaging and Radiological Sciences, National Yang Ming Chiao-Tung University, Taipei, Taiwan.


Shi-Long Chang
Department of Pathology, Tao-Yuan General Hospital, Ministry of Health and Welfare, Tao-Yuan, Taiwan.

Jyh-Der Leu
Division of Radiation Oncology, Taipei City Hospital RenAi Branch, Taipei, Taiwan.


Yu-Chan Chang
Genomic Research Center, Academia Sinica, Taipei, Taiwan.

Michael Hsiao
Genomic Research Center, Academia Sinica, Taipei, Taiwan.

Liang-Ting Lin
Department of Health Technology and Informatics, The Hong Kong Polytechnic University, Kowloon, Hong Kong.

Han-Nan Lin
Department of Hematology and Oncology, Tao-Yuan General Hospital, Ministry of Health and Welfare, Tao-Yuan, Taiwan.

Yi-Jang Lee
Department of Biomedical Imaging and Radiological Sciences, National Yang Ming Chiao-Tung University, Taipei, Taiwan and Cancer Progression Research Center, National Yang Ming Chiao Tung University, Taipei, Taiwan.

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