Assessment of Isoniazid and Pyridoxine Hydrochloride in Pure and Pharmaceutical Preparations


For the simultaneous assay of pyridoxine hydrochloride and isoniazid, a new spectrophotometric approach was devised. The approach was based on a proton transfer reaction between pyridoxine hydrochloride B6 and chromeazurol S at pH = 5.7, which produced a red, water soluble compound that absorbed at 510 nm. This technique for detecting isoniazid IND was discovered by bleaching the red-colored product, pyridoxine hydrochloride –chromeazurol S. Numbness and elevated blood levels of liver enzymes are frequent adverse effects of the IND. At concentrations ranging from 5 to 700 g/25ml for pyridoxine hydrochloride (i.e. 0.2-28.0) ppm, the molar absorptivity value was 1.51104 l/, while at concentrations ranging from 25 to 1500 g/25ml for isoniazid (i.e. 1-60) ppm, the value of molar absorptivity was 1.36104 l/ This research seeks to develop a spectroscopic technique for determining isoniazid and pyridoxine hydrochloride in pharmaceutical formulations. As a result, this method has been successfully employed to detect pyridoxine hydrochloride and isoniazid in pharmaceutical formulations.

Author(s) Details:

Basima A. A. Saleem,
Department of Chemistry, College of Science, Mosul University, Mosul, Iraq.

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