Assessment of Isoniazid and Pyridoxine Hydrochloride in Pure and Pharmaceutical Preparations
For the simultaneous assay of pyridoxine hydrochloride and isoniazid, a new spectrophotometric approach was devised. The approach was based on a proton transfer reaction between pyridoxine hydrochloride B6 and chromeazurol S at pH = 5.7, which produced a red, water soluble compound that absorbed at 510 nm. This technique for detecting isoniazid IND was discovered by bleaching the red-colored product, pyridoxine hydrochloride –chromeazurol S. Numbness and elevated blood levels of liver enzymes are frequent adverse effects of the IND. At concentrations ranging from 5 to 700 g/25ml for pyridoxine hydrochloride (i.e. 0.2-28.0) ppm, the molar absorptivity value was 1.51104 l/mol.cm, while at concentrations ranging from 25 to 1500 g/25ml for isoniazid (i.e. 1-60) ppm, the value of molar absorptivity was 1.36104 l/ This research seeks to develop a spectroscopic technique for determining isoniazid and pyridoxine hydrochloride in pharmaceutical formulations. As a result, this method has been successfully employed to detect pyridoxine hydrochloride and isoniazid in pharmaceutical formulations.
Basima A. A. Saleem,
Department of Chemistry, College of Science, Mosul University, Mosul, Iraq.
Please see the link here: https://stm.bookpi.org/PCSR-V1/article/view/6769