Obligate anaerobic bacteria play a pivotal role in humans’ microbiota and are essential infectious agents, both in immunocompetent and immunocompromised persons, involved in several pathological processes. Their isolation, cultivation and accurate identification vary greatly from the work of aerobic organisms, while anaerobic diagnostics have improved drastically with the use of new technologies ( e.g., matrix-assisted laser desorption / ionisation time-of – flight mass spectrometry, whole genome sequencing). For these reasons, for surveillance purposes or otherwise, antimicrobial susceptibility testing of anaerobic isolates is of vital importance, however the availability of these testing methods is generally restricted. Our objective in this chapter was to give an overview of the currently available methods for testing for antimicrobial susceptibility (agar dilution, broth microdilution, disc diffusion, gradient testing, automated systems, detection of phenotypic and molecular resistance). detection techniques) of anaerobes, when should these methods be used and what are the recent developments in resistance patterns of anaerobic bacteria.
Author (s) Details
Institute of Translational Medicine, Faculty of Medicine, University of Pécs, 7624 Pécs, Szigeti út 12., Hungary.
Department of Pharmacodynamics and Biopharmacy, Faculty of Pharmacy, University of Szeged; 6720 Szeged, Eötvös utca 6., Hungary and Institute of Microbiology, Faculty of Medicine, Semmelweis University; 1089 Budapest, Nagyvárad tér 4., Hungary.
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