News Release on Microbiome Research: February-2019

Innate immunity and oral microbiome: a customized, predictive, and preventive approach to the management of oral diseases

Three recent advances in medicine, genetics, and biology have ushered in an exceedingly new era within the continued  efforts to higher perceive and treat oral diseases, moving ever nearer to the 3 note of recent healthcare: personalised, predictive, and practice of medicine (PPPM). the invention of currently fifteen subtypes of innate liquid body substance cells, the refinement of polymer sequencing, and culture-independent characterization of the whole microorganism community begin to reveal this complicated adjustive network. of these advances warrant a scientific review as they need modified and can still amendment medical specialty. we are going to update dental professionals on these advances as associated with oral diseases and associated pathologies in different organ systems like labour, arthrosclerosis, and cancer. [1]

RE: “QUANTIFICATION OF HUMAN MICROBIOME STABILITY OVER vi MONTHS: IMPLICATIONS FOR medical specialty STUDIES”

We browse with keen interest the paper by Sinha et al. (1), during which they evaluated phylum-level microbiome stability across pairs of samples obtained six months other than three separate studies. All three studies had replicate faecal samples (n = 44–116 replicates), a pair of studies had replicate spittle samples (n = 42–94 replicates), and one study had replicates from four further body sites. Sample assortment, processing, and 16S ribosomal polymer sequencing ways differed between studies. The authors used intraclass correlation coefficients (ICCs) to judge microbiome stability, examination phyla relative abundances, α-diversity measures (number of operational taxonomical units, Chao1,… [2]

Insights into microbiome analysis 6: The role of consortia in learning the role of microbes in health and malady

Consortia are giant, multidisciplinary analysis efforts that carry the ability of numbers. additionally, they allow the sharing of protocols and methods, ultimately leading to credible, duplicatable, and groundbreaking discoveries. many consortia are presently work the role of bacterium in health and illness, as well as the International disseminated multiple sclerosis Microbiome Study (iMSMS), whose effort is devoted to disseminated multiple sclerosis [3]

Effect of breastfeeding versus pumping on human milk microbiome

A large-scale analysis in humans suggests that the milk microbiota is tormented by microorganism each from the infant’s mouth and from environmental sources like breast pumps, though future analysis are going to be required to assess the consequences that these changes might wear the child gut microbiome and infant health. [4]

Effects of penicillin on the Gut Microbiome of an man as Determined by 16S rRNA V4 Metagenomics Sequencing and Greengenes Bioinformatics Suite

Aims: to see the results of penicillin on the relative abundance of the gut microbiota mistreatment Next Generation Sequencing (NGS) metagenomics platform and to predict metabolic functions with bioinformatics suite.

Methods: the topic UN agency has taken no antibiotics for the past six months provided unclean sample on day zero (pre-antibiotics), day ten (antibiotics) to treat bitter streptococcal sore throat and when ninety days (post-antibiotics). polymer was extracted, metagenomics library ready and 16S rRNA V4 region was amplified mistreatment custom bar-coded primers before sequencing with IlluminaMiseq program. Sequence reads were analyzed with IlluminaBasespace formula and greengenes bioinformatics suites. Metabolic purposeful prediction was accomplished mistreatment the biological process Investigation of Communities by Reconstruction of Unobserved States (PICRUSt) formula. [5]

Reference

[1] Jack CY, Khodadadi H, Baban B. Innate immunity and oral microbiome: a personalized, predictive, and preventive approach to the management of oral diseases. EPMA Journal.:1-8. (web link)

[2] McSkimming DI, Banack HR, Genco R, Wactawski-Wende J, LaMonte MJ. RE:“QUANTIFICATION OF HUMAN MICROBIOME STABILITY OVER 6 MONTHS: IMPLICATIONS FOR EPIDEMIOLOGIC STUDIES”. American Journal of Epidemiology. (web link)

[3] Baranzini SE. Insights into microbiome research 6: The role of consortia in studying the role of microbes in health and disease. Multiple Sclerosis Journal. 2019 Mar;25(3):336-7. (web link)

[4] Effect of breastfeeding versus pumping on human milk microbiome

Date: February 13, 2019

Source: Cell Press (Web link)

[5] Effects of Ampicillin on the Gut Microbiome of an Adult Male as Determined by 16S rRNA V4 Metagenomics Sequencing and Greengenes Bioinformatics Suite

Kingsley Anukam

Uzobiogene Genomics, London, ON, Canada (web link)

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